Platelet aggregation by LTA

Suspension of platelets or decoys in plasma was recalcified with CaCl₂ at a final concentration of 1 mM (36). LTA after addition of agonists (ADP, TRAP, and collagen) was measured under magnetic stirring at 37°C using a Chrono-log instrument. Specifically, platelet control groups used platelets extracted from the same blood donor via centrifugation steps and resuspended in PPP. In P:D groups, platelet decoys were added in addition to normal platelet samples. For example, for a P:D ratio of 1:0.2, we added 20% decoys in addition to 100% platelets for P:D sample, whereas the same volume of PPP was added in the platelet control (100% platelets) to account for volume expansion.