Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

G-LISA Rac1, RhoA, and Ras activation assays

DM De Yu Mao
MK Matthew L. Kleinjan
IJ Irina Jilishitz
BS Bhairavi Swaminathan
HO Hideru Obinata
YK Yulia A. Komarova
KB Kayla J. Bayless
TH Timothy Hla
JK Jan K. Kitajewski
ask Ask a question
Favorite

Assays were performed using Rac1 (Cytoskeleton, BK128), RhoA (Cytoskeleton, BK124), and Ras (Cytoskeleton, BK131) G-LISA Activation Assay Kits. HUVECs were serum-starved in EBM-2 with 1% serum overnight and with serum-free EBM-2 for an additional 3 hours the following day. The cells were then stimulated with 1 μM S1P for Rac1, RhoA, and Ras activation. Cell lysates were harvested and snap-frozen in liquid nitrogen. The assay was then performed on the basis of the manufacturer’s protocol.

Copyright and License information:  ©2021

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A