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Subcellular localization

RS Romana Schober
DB Daniel Bonhenry
VL Victoria Lunz
JZ Jinhui Zhu
AK Adela Krizova
IF Irene Frischauf
MF Marc Fahrner
MZ MengQi Zhang
LW Linda Waldherr
TS Tony Schmidt
ID Isabella Derler
PS Peter B. Stathopulos
CR Christoph Romanin
RE Rüdiger H. Ettrich
RS Rainer Schindl
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The same technical equipment as for confocal FRET microscopy was used. ImageJ was used to analyze subcellular localization of transcription factors by intensity measurements of the cytosol and nucleus. Three different populations were distinguished with different nucleus/cytosol ratios: inactive (<0.85), homogenous (0.85 to 1.15), and active (>1.15).

All data are presented as means ± SEM for the indicated number of experiments. Statistical significance was determined by unpaired two-sided Student’s t tests for comparison of two groups (using OriginPro 2017). Statistical significance was set to P < 0.05.

Copyright and License information:  ©2019-11-19

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