Analysis of S. pombe cell length at division and CDC25-GFP localization

Exponentially growing S. pombe were resuspended in PBS and mounted onto poly-l-lysine coated slides. Differential interference contrast images were taken using a Zeiss Axioscope, and cell lengths were compared by measuring >94 newly divided cells for each sample. For analysis of fluorescence (CDC25-GFP localization), cells were mounted in VECTASHIELD containing 4′,6-diamidino-2-phenylindole (DAPI) (1.5 μg/ml) to visualize DNA and observed on a Zeiss Axioscope fluorescence microscope using appropriate filters.