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FAP and type I collagen quantification

HM H. Patrick MacKnight
DS Daniel J. Stephenson
LH L. Alexis Hoeferlin
SB Savannah D. Benusa
JD James T. DeLigio
KM Kenneth D. Maus
AA Anika N. Ali
JW Jennifer S. Wayne
MP Margaret A. Park
EH Edward H. Hinchcliffe
RB Rhoderick E. Brown
JR John J. Ryan
RD Robert F. Diegelmann
CC Charles E. Chalfant
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FAP and type I collagen quantification were performed via the following process: Images were loaded into Adobe Photoshop, and background was subtracted as follows: blue (0000FF), purple (800080), and light blue (ADD8E6). Following background subtraction, new images were loaded in Fiji ImageJ bundle and converted into 8-bit images. Thresholds for these images were set using the auto selection option in ImageJ. Last, images were analyzed using the analyze particles tool in ImageJ.

Copyright and License information:  ©2019-12-03

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