CFA-induced inflammatory pain

Mice received 20 μl of CFA (1 mg/ml; Sigma, St. Louis, MO) in the right hind paw, as previously described (61). They were acclimated to the dynamic plantar aesthesiometer testing chamber for 1 hour, two consecutive days before behavioral testing. From days 4 to 14 after CFA injection, mice received either 10 μl of PBS or Nal-M (2 mg/ml) in the ipsilateral ankle. Mechanical threshold was assessed 30 min after injection. Paw edema was measured throughout the experiment with a plethysmometer (Hugo Basile).