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Gene ontology (GO) and KEGG pathway enrichment analysis of differentially expressed genes (DEGs)

ZZ Zi-Xin Zhang
SZ Shu-Niu Zhao
GL Gao-Feng Liu
ZH Zu-Mei Huang
ZC Zhen-Mu Cao
SC Shan-Han Cheng
SL Shi-Sen Lin
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The Gene Ontology database (http://www.geneontology.org/) classifies genes into three general categories including “cellular component,” “molecular function” and “biological process”17. It can be used to predict the biological function of DEGs after mapping the sequencing reads to reference genes. After annotating expressed genes with GO terms, a GO functional classification was performed with WEGO.

All DEGs were mapped to terms in the KEGG database. Genes with a P-value ≤ 0.05 were considered to be differentially expressed. KEGG was used to graphically represent biological pathways and to display the up- or down-regulation of genes in a pathway.

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