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Transfected cells were seeded in 96-well microplates. Activities of caspase-3 and caspase-7 together with cell viability were measured 24 and 72 hours after transfection using the Caspase-Glo 3/7 Assay Kit (Promega) and ATPlite 1step Luminescence Assay System (PerkinElmer Life Sciences) according to the manufacturer’s instructions. Luminescence was measured using an EnSpire 2300 Multilabel Reader (PerkinElmer Life Sciences). Three to four independent biological experiments, each prepared in triplicate or quadruplicate for each transfection condition, were performed with reproducible results.
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