An antibody recognizing TNFR2 (TR75-54.7) and/or an antibody recognizing CD25 (PC61) were administered at 200 μg in 0.2 ml of PBS 3 days before the inoculation of 1 × 105 4T1 breast tumor cells in 0.1 ml of PBS per mouse subcutaneously injected into right mammary fat pads (thoracic No. 2 mammary glands) of recipient female Balb/c mice.
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