Treatment of mouse model of CT26 colon cancer

When the diameter of tumor reached 5 to 6 mm, mice were treated with the following dose schedule: An antibody recognizing TNFR2 (M861) or mouse IgG1 was administered at days 1, 5, and 9 intraperitoneally at 100 μg in 0.2 ml of PBS. CpG ODN or control ODN was administered intratumorally at days 2 and 4 at 20 μg in 0.1 ml of PBS. The same quantity of PBS was administered intraperitoneally or intratumorally as a control. In some experiments, the treatment was started when the diameter of tumor reached 10 mm. One day after the last treatment, mice were sacrificed and tumor and lymphoid tissues were harvested for study.