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Electrophoretic mobility shift assay

SS Sunil Kumar Saini
TT Tripti Tamhane
RA Raghavendra Anjanappa
AS Ankur Saikia
SR Sofie Ramskov
MD Marco Donia
IS Inge Marie Svane
SJ Søren Nyboe Jakobsen
MG Maria Garcia-Alai
MZ Martin Zacharias
RM Rob Meijers
SS Sebastian Springer
SH Sine Reker Hadrup
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After biotinylation, purified MHC-I monomers were tested for biotinylation efficiency. Purified monomers (2 μg) were incubated with 4 μg of Streptavidin-plus (ProZyme SA10) for 30 min at 4°C. After incubation, proteins were separated on 4 to 20% Mini-PROTEAN TGX Gel (Bio-Rad, 4561095) along with a PAGE ruler prestained protein ladder (Bio-Rad, 1610374) and stained with Coomassie (Bio-Rad, 161-0786).

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