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Plasmids encoding Dc1a mutants were generated via PCR-based mutagenesis using a plasmid encoding wild-type Dc1a [pLIC-NSB3; (10)] as template. The DNA sequence of all mutants was confirmed by Sanger sequencing. Peptide concentrations were determined by calculating the area under the RP-HPLC peak (at 214 nm) of all analogs, then comparing these to the peak area obtained from a Dc1a standard whose concentration had been determined by amino acid analysis.
Copyright and License information: ©2018-10-19
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