Cisplatin treatment in mice

Ten milligrams of cisplatin (479306, Sigma-Aldrich) powder was dissolved in 10 ml of sterile saline (0.9% NaCl) at 37°C for 40 to 60 min. Cisplatin was administered to FVB mice at 30 mg/kg via intraperitoneal injection. One day before cisplatin injection, mice received 1 ml of saline by subcutaneous injection. To ameliorate dehydration after cisplatin injection, 1 ml of warm saline was injected twice per day for at least 7 days or until body weight started to recover. The cages of cisplatin-treated mice were placed on the heating pad for at least 7 days or until body weight began to recover. Fresh mush food was daily given for at least 7 days. A survival rate of 50% was observed in both cisplatin and combined cisplatin with dabrafenib treatments.