Q5 Site-Directed Mutagenesis

C. Cassart; C. Yague-Sanz; F. Bauer; P. Ponsard; F. X. Stubbe; V. Migeot; M. Wery; A. Morillon; F. Palladino; V. Robert; D. Hermand

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Q5 Site-Directed Mutagenesis

CC C. Cassart

CY C. Yague-Sanz

FB F. Bauer

PP P. Ponsard

FS F. X. Stubbe

VM V. Migeot

MW M. Wery

AM A. Morillon

FP F. Palladino

VR V. Robert

DH D. Hermand

This method is extracted from research article: Sci Adv, Dec 2020

RNA polymerase II CTD S2P is dispensable for embryogenesis but mediates exit from developmental diapause in C. elegans

DOI: 10.1126/sciadv.abc1450

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The Q5 Site-Directed Mutagenesis was made using the Q5 Site-Directed Mutagenesis Kit of New England Biolabs (E0554S) following the instructions of the manufacturer (Q5 Site-Directed Mutagenesis Kit Quick Protocol, E0554). The primers were designed with NEBaseChanger.

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