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Cell proliferation was determined by a 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. 1 × 105 cells were seeded in each well of a 96-well plate (BD Falcon, Franklin, NJ) and allowed to adhere for 8 h. MTT solution was then added to each well at the final concentration of 0.5mg/mL and incubated for 4 hours at 37°C. After removing the culture medium, 150μL of dimethyl sulfoxide (DMSO), sunitinib and celecoxib were added respectively, and the optical density of each well was measured by spectrophotometer at 495 nm.
Copyright and License information: ©2017 Zhao et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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