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cDNA generation for targeted RNA amplicon sequencing
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cDNA generation was performed with SuperScript IV (Thermo Fisher Scientific) according to the manufacturer’s instructions. A poly-T primer was used to selectively amplify mRNAs in the cDNA synthesis step. The optional step of RNase degradation before amplification of cDNAs was included to improve the efficiency of PCR. We note that this step was particularly important for RSL1D1 PCR.
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