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Resazurin reduction capacity

SA Sofia Abreu
FS Fernanda Silva
RM Rita Mendes
TM Teresa F. Mendes
MT Marta Teixeira
VS Vítor E. Santo
EB Erwin R. Boghaert
AF Ana Félix
CB Catarina Brito
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Resazurin reduction capacity of cells present in the explants was assessed using the PrestoBlue Cell Viability Reagent (A13262, Invitrogen). The active ingredient of PrestoBlue reagent (resazurin) is a non-toxic and non-fluorescent dye, that when in contact with a viable cell is reduced, becoming red-fluorescent resorufin76. At days 0, 10, 20 and 30 of each OvC-PDE culture analysed (N = 7–8), 1 mL of culture suspension (on average, 5 explants) was collected in triplicate, and incubated with PrestoBlue reagent (diluted 1:10) during 1 h at 37 °C. After this, supernatants of triplicates were collected to a 96-well black fluorescence reading plate (Corning) and fluorescence reading was performed (Infinite 200 PRO NanoQuant plate reader, TECAN), setting the excitation wavelength at 570 nm and emission wavelength at 590 nm.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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