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Briefly, at a density of 1 ×10 4 cells/well THP-1 human macrophage or PBMCs were plated into 96-well plates. After the applied LPS treatment, MTT dye (5 mg/mL, 20 μL in each well) was added. After incubation for another 2-3h, The optical density (OD) of MTT at 490 nm was recorded.
Copyright and License information: ©2020 Sun et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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