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S. marcescens was grown in the absence or presence of indole or indole derivatives at 30°C for 20 h and centrifuged at 12,000 rpm for 10 min. Acidified ethanol (1 mL of 4% 1 M HCl in ethanol) was added to the cell pellets obtained and vortexed vigorously to extract prodigiosin. After centrifugation, supernatant absorbances were measured at 534 nm as previously reported (Slater et al., 2003).
Copyright and License information: ©2020 Sethupathy, Sathiyamoorthi, Kim, Lee and Lee.
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