Prognostic Analysis via GEPIA2 and the Kaplan Meier Plotter

Similar to the GEPIA database, GEPIA2 (http://gepia2.cancer-pku.cn/) is an updated version that provides insights with higher resolution and more features.¹⁸ Pancreatic cancer patients were categorized into high-GINS expression and low-GINS expression groups based on the median expression values of the GINS complex, each group contained 89 samples. Subsequently, we compared the overall survival (OS) and disease-free survival (DFS) difference between the two groups using the Kaplan Meier survival plot. This procedure was purposed to identify whether the whole GINS complex could be considered as a prognostic signature in pancreatic cancer. We employed the Kaplan Meier Plotter (www.kmplot.com), an online prognostic analysis platform which analyzes the prognostic value of genes in 21 common cancer categories,¹⁹ to analyze the prognostic value of each individual in the GINS complex in pancreatic cancer following the results obtained by the OS and recurrence-free survival (RFS) difference. Using the median expression value, we categorized pancreatic cancer patients into high or low expression group. This procedure aimed to identify the prognostic value of each individual in the GINS complex in pancreatic cancer. Hazard ratio (HR) and p-value were depicted in every single plot.