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Inhibition assay

ZA Zahra Alvandi
MO Michal Opas
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c-Src inhibitor PP2 (Calbiochem, Catalog No. 529573) and negative control PP3 (Calbiochem, Catalog No. 529574) were applied at concentrations described by us previously [26, 27] and in ES cells specifically [28] based on survey by Bain et al. [29] wherever indicated. For multiple days treatment, media was replaced with fresh inhibitor containing media every day during the treatment period. For protein half-life assay, Cycloheximide (Sigma-Aldrich, Catalog No. C7698) was applied at 10 μM concentration for the indicated times. Proteasome inhibitor MG132 was purchased from Millipore (Catalog No. 474790).

Copyright and License information:  ©2020 Alvandi, Opas
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