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To confirm that ST6Gal-I knockdown corresponded with a decrease in α2–6 sialic acid on the cell surface, staining was performed with SNA lectin, which binds to α2–6 sialic acid. Briefly, cells were incubated with FITC-conjugated SNA (Vector, B-1305) at a 1:400 dilution for 20 minutes at 4°C. SNA staining was then quantified via flow cytometry using an LSR-II (BD Bioscience).
Copyright and License information: ©2020 Holdbrooks et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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