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The cells were centrifuged at 1000 rpm for 5 min, washed twice with PBS and then centrifuged at 1000 rpm for 5 min. Then, the cells were collected then added 100 µL binding buffer to prepare a single-cell suspension with a density of 2×105/mL. 5 µL Annexin V-APC and 5 µL 7-AAD (Becton Dickinson) were added per tube and mixed thoroughly. Each mixture was then incubated at room temperature for 10 min and then detected using flow cytometry. Empty cell controls were set and the above procedures were repeated.
Copyright and License information: ©2020 Chen et al.
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