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Cells grown in logarithmic phase were inoculated in 96-well plates at a density of 1 × 104 cells/well for conventional culture. After 24 hours, 10 μl CCK-8 solution was added to each well. Cells were placed in the dark for 1 hour, then absorbance at a 450-nm wavelength was detected by a microplate reader (Bio-Rad, Hercules, CA, USA).
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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
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