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L-KYN and KYNA were obtained from Sigma Aldrich (St. Louis, MO, USA). L-KYN was dissolved in cell culture medium, whereas KYNA was dissolved in 1 N NaOH, and then phosphate buffered saline (PBS). FICZ, obtained from Tocris Bioscience (Bristol, UK), was dissolved in dimethyl sulfoxide (DMSO). The final concentration of DMSO in cell culture medium dilutions was less than 0.2%. No significant effects of solvents on melanoma and melanocyte cell proliferation and morphology were observed.
Copyright and License information: ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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