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Lentivirus production and infection

LX Linguo Xu
LH Lin Huan
TG Tianan Guo
YW Yangjun Wu
YL Yanfang Liu
QW Qifeng Wang
SH Shenglin Huang
YX Ye Xu
LL Linhui Liang
XH Xianghuo He
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Full-length SNHG11 was generated by RACE experiments and then was cloned into a pCDH-Puro lentiviral vector. The ORF of HIF-1α was cloned into the pCDH-3×Flag lentiviral vector. The primers used to amplify the sequences are listed in Supplementary Table 8. shRNA sequences were synthesized and subcloned into a LentiGuide-Puro lentiviral vector. shRNA sequences are listed in Supplementary Table 8. To generate lentiviruses, pAX2 and pMD2.G were cotransfected into HEK293T cells using Lipofectamine 2000 (Invitrogen, CA, USA). After 48 h, lentiviruses were collected and used to infect CRC cells.

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