Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Western blot and confocal microscopy analysis of the P16 expression status
Request a Protocol
Ask a
question
Favorite
The P16 mRNA and protein levels in the cells were analysed as previously described [19]. Rabbit monoclonal antibody against human P16 protein (ab108349, Abcam, Britain) was used in the Western blot assay, and mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) was used in the immunostaining assay. These experiments were performed in triplicate and repeated at least one time.
Copyright and License information: ©2019 Informa UK Limited, trading as Taylor & Francis Group
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
