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Scratch test

YG Yang Gu
SZ Shulan Zhang
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Even horizontal linear scratches were made in the back of 6-well plate by a marker pen with 3 scratches in each well. Six pairs of cells at logarithmic growth phase were seeded in the culture plate (5 × 105 cells/well). Following 24-h culture, when cells settled at the bottom of the well, 200 μL pipette tips was adopted to make even vertical linear scratches in the back of the plate with 3 scratches in each well. Afterwards, cell incubation was conducted in RPMI-1640 medium with 5% CO2 at 37 °C. The migration distance was observed and photographed under inverted microscope at 0 h and 24 h after scratch. Three replicates were made in each group. The number of cells passing through scratches was considered as an indicator of the migration ability.

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