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De-paraffinized and rehydrated tissue sections were stained with hematoxylin and then dipped in 5% sodium thio-sulphate solution for 2 min. Then the sections were washed with tap water followed by 9:1 saturated Picric acid: acid fuschin 2 min treatment. Further, tissue sections were washed with water followed by alcohol gradients before mounting into DPX.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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