Methylated RNA Immunoprecipitation (MeRIP) Assay

The MeRIP assay was conducted by using the Magna MeRIP m6A Kit (17–10,499, Millipore, USA) according to the manufacturer’s instructions. Briefly, 5 μg isolated mRNA was chemically fragmented into 100 nucleotides or less and immunoprecipitated with 5 μg m6A antibody or anti-mouse IgG linked to Magna IP Protein A/G Magnetic Beads. One tenth volume of fragmented RNA was saved as “10% input.” After elution with 6.7 mM N6-methyladenosine 5′-monophosphate sodium salt, the expression of m6A-modified genes was determined by qRT-PCR with specific primers whose sequences are shown in Table S3. Then, qRT-PCR products were further analyzed via agarose gel electrophoresis (AGE).