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Analysis of caspase-1 activation by western blot
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The 14-week-old ApcMin/+ mice were immunized with VLP-hCEA orally. After 24 and 48 h, intestinal tumour polyps were collected and lysed for cellular protein extraction. Protein extractions were then subjected to SDS–polyacrylamide gel electrophoresis, electroblotted to a nitrocellulose membrane and immunoblotted against mouse caspase-1 p20 fragment (AdipoGen) or anti β-actin (Biolegend).
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