Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Transcriptome analysis by RNA-Seq

JL Jeong Gu Lee
GY Gibum Yi
JS Jieun Seo
BK Byoung-Cheorl Kang
JC Jeong Hee Choi
EL Eun Jin Lee
request Request a Protocol
ask Ask a question
Favorite

Total RNA from ‘UZB-GJG-1999-51’ and ‘C00562’ peppers treated with chilling for 0 h and 24 h were used for RNA-seq. RNA-seq was performed using the Illumina Hi-Seq 2500 system (Illumina, San Diego, CA, USA) with 151-bp paired-end at the National Instrumentation Center for Environmental Management (NICEM), Seoul National University, South Korea. As a pretreatment process, artifacts such as adaptor sequence, contaminant DNA, and PCR duplicates, were removed from raw reads. Then, preprocessed reads were assembled and mapped to Pepper Zunla 1 Ref_v1.0 reference genome (GCF_000710875.1, NCBI).

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A