Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Antiviral activity assay using native SARS-CoV-2 virus
Ask a
question
Favorite
SARS-CoV-2 (WIV04) [18] was passaged in Vero E6 cells and titered by a plaque assay. Vero E6 cells were treated with drugs at the indicated concentrations and exposed to SARS-CoV-2 virus at an MOI of 0.01. Cells cocultured with virus were maintained in DMEM with 2% FBS, and the supernatants were collected at 24 h post infection. Magnetic Bead Virus RNA Extraction Kits (Shanghai Fine Gene Biotech, FG438) were used for viral RNA extraction. Viral RNA was quantified by real-time RT-PCR with TaqMan probes targeting the RBD2 gene.
Copyright and License information: CPS and SIMM ©2020
This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
