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Plant Terpenoids Extraction

XF Xin Fang
CL Chen-Yi Li
YY Yue Yang
MC Meng-Ying Cui
XC Xiao-Ya Chen
LY Lei Yang
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The following materials from the 6-month-old plants in bloom were harvested for extraction of chemicals: primary root (6 cm length from the apical); stem (the second to the fourth internodes from the top); the third pair of true leaves; and the inflorescence. The 6-month-old plants contained a high level of terpenoids and suitable for extraction.

Fresh materials (0.5 g) were ground in liquid nitrogen, followed by adding 2.5 mL hexane to extract the terpenoids in a shaker at 30°C for 1 h. A short column of aluminum oxide overlaid with anhydrous MgSO4 was used to purify the extracts, and the obtained hexane phase was analyzed by Gas chromatography–mass spectrometry (GC-MS) by the total ion chromatogram mode.

Copyright and License information:  ©2017 Fang, Li, Yang, Cui, Chen and Yang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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