4.3. Measurement of Cell Viability by MTT Assay

PANC-1 cells were plated on a 96-well plate at 1 × 10⁵ cells/mL and incubated. After 24 h, the medium was changed and treated with various concentrations of the samples. Control cells were treated with DMSO. After incubation of the cells with samples for 48 h, 20 μL of 1 mg/mL MTT solution was added to each well and incubated at 37 °C for four hours. The supernatant was then removed and 150 μL DMSO was added to each well to dissolve the formazan. The formazan formation was measured with a microplate reader at a wavelength of 570 nm and a reference wavelength of 655 nm.