Mice

WT C57BL/6 (#000664), WT C57BL/6N (#005304), Casp1^−/− (#032662), Gsdmd^−/− (#032410), Stat2^−/− (#023309), Casp1^−/− Casp11^−/− (#016621), and Cas9 knockin (#026179) mice (all in C57BL/6 background) were purchased from The Jackson Laboratory. Casp11^−/− mice were kindly provided by J. Blander. Cas9 knockin mice were mated with WT C57BL/6 mice, and the resulting offspring carrying one Cas9 allele (Cas9-het) were used for electroporation of gRNA amplicons. Female 129S2 mice (#476) were purchased from Charles River Laboratories, rested for 2 wk, and then infected at the age of 8 wk. All mice were housed in a specific pathogen-free facility. When infected with Mtb, mice were housed in a BSL3 vivarium. All mouse experiments were approved by and performed in accordance with requirements of the Weill Cornell Medicine Institutional Animal Care and Use Committee.