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Cells were lysed with IP lysis buffer (P0013, Beyotime). The whole-cell lysates were incubated with antibodies overnight at 4 °C and then precipitated with the antibody-protein complex, using Protein A/G beads (Thermo Fisher Scientific). The immunoprecipitates were washed five times and then subjected to western blotting analysis.
Copyright and License information: ©2020 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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