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Cells were grown on glass coverslips, fixed and permeabilized. PLA (Duolink) was performed according to the manufacturer's (Olink) instructions using antibodies against BRAF (sc5284, Santa Cruz, 1:500) and NRAS (sc519, Santa Cruz, 1:50), or CRAF (#610151, BD Biosciences, 1:50) and NRAS. Knockout cells for BRAF or CRAF were used as control. Images were captured using a 3D/optigrid Leica fluorescent microscope. The average number of dots per cell (identified by its nucleus) was determined by analysing at least 70 different cells with the ImageJ software.
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