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2.2. Determination of Chlorophyll Content in Plant
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The chlorophyll content was assayed spectrophotometrically. Fresh leaves (0.2 g) were added to a tube containing 2 mL of 100% acetone. After shaking for 24 h at 4 °C, the absorbance was read at 645 nm and 663 nm using a spectrophotometer to determine chlorophyll content [21].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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