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Neon Transfection System

EL Elaine A. Liu
MS Mark L. Schultz
CM Chisaki Mochida
CC Chan Chung
HP Henry L. Paulson
AL Andrew P. Lieberman
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Cells were transfected by Neon Transfection System (Thermo Fisher Scientific) according to manufacturer’s protocol. Briefly, cells were counted and resuspended in DPBS (Gibco) along with plasmid DNA. Cell culture plates were preincubated with culture medium without antibiotics. Neon Tube was set up with 3 mL Electrolytic Buffer E2 and the 100 μL Neon Tip was used at 1200V, 40 ms, 1 pulse.

Copyright and License information:  ©2020 Liu et al.
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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