Sorafenib-resistant cells

Initially, we examined the 50% inhibiting concentration (IC₅₀) of HepG2 and Huh-7 cells following sorafenib treatment. Subsequently, we seeded HepG2 and Huh-7 cells in six-well plates at a density of 1 × 10⁴ cells per well and incubated the cells with sorafenib concentrations just below their respective IC₅₀. During the following time periods, the concentration of sorafenib was slowly increased by 0.25 μmol/L per week. Following a 6-month treatment period, the two cell lines resistant to sorafenib were established and were named HepG2 SR and Huh7 SR. The cells were continuously cultured in the presence of sorafenib. Finally, we incubated HepG2, Huh7, HepG2-SR, and Huh7-SR cells with gradually increasing doses of sorafenib in 96-well plates and determined the cell viability by the MTT assay following 2 days of incubation.