Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Transfection Assays

XY Xue Yang
LN Li Niu
YP Yaping Pan
XF Xianghui Feng
JL Jie Liu
YG Yan Guo
CP Chunling Pan
FG Fengxue Geng
XT Xiaolin Tang
request Request a Protocol
ask Ask a question
Favorite

For transfection, the HaCaT cells were plated on six-well, flat-bottom plates at a seeding density of 3 × 105, and grown to 80% confluence. For transient overexpression of LL-37, the LL-37 plasmid was transfected into HaCaT cells for 6 h. An empty vector was used as the control. The instructions to complete cell transfection were followed. Then, we replaced the medium with fresh serum-free α-MEM. The cells transfected with plasmids were used in subsequent experiments.

Copyright and License information:  ©2020 Yang, Niu, Pan, Feng, Liu, Guo, Pan, Geng and Tang.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A