Anti-Flag M2 agarose beads (A2220) and anti-HA agarose beads (A2095) were purchased from Sigma. Anti-Chk1 (sc-56288) and anti-p53 (sc-55476) antibodies were purchased from Santa Cruz. Anti-PARP-1 (9532), anti-pChk1S345 (2341), anti-pChk1S317 (2344), anti-p-p53 (9284), anti-γ-H2AX (9718), anti-pATR-S428 (2853), anti-pATM-S1981 (13050) and anti-pCdc25C (9528S) antibodies were purchased from Cell Signaling Technologies. Cycloheximide (C4859), cisplatin (479306), and imidazole (12399) were purchased from Sigma. Ni-NTA resin (635659) was purchased from Clontech.
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