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2.3. UV/vis and Fluorescence Spectroscopic Methods
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All the UV/Vis absorption spectra and fluorescence were recorded on S-3100 and RF-5301PC spectrophotometer (Shimadzu, Kyoto, Japan), respectively. Stock solutions (500 μM) of probes were prepared in DMSO, and then diluted into 10 μM solution of probes in PBS buffer with 2% DMSO. In all experiments, the excitation wavelength was 648 nm and the emission wavelength was 673 nm.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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