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3.5. Cyclooxygenase (COX-2) Inhibition Assay

JH Jianxin Han
WL Wei Liu
ML Miaomiao Li
YG Yanpei Gu
YZ Ying Zhang
TY Tao Yuan
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The COX-2 inhibitory activity study was carried out using ovine recombinant COX-2 enzyme by a COX colorimetric inhibitor screening assay kit from Cayman Chemical Co. (Ann Arbor, MI, USA). The assay was performed following the protocol provided by the manufacturer of assay kit. Non-selective inhibitor, naproxen, was used as a positive control. The COX inhibitory activity was expressed as % inhibition and was calculated as following Equation:

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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