Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

3.5. Cyclooxygenase (COX-2) Inhibition Assay

JH Jianxin Han
WL Wei Liu
ML Miaomiao Li
YG Yanpei Gu
YZ Ying Zhang
TY Tao Yuan
request Request a Protocol
ask Ask a question
Favorite

The COX-2 inhibitory activity study was carried out using ovine recombinant COX-2 enzyme by a COX colorimetric inhibitor screening assay kit from Cayman Chemical Co. (Ann Arbor, MI, USA). The assay was performed following the protocol provided by the manufacturer of assay kit. Non-selective inhibitor, naproxen, was used as a positive control. The COX inhibitory activity was expressed as % inhibition and was calculated as following Equation:

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A