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NCI-H1975 and PC9/GR cells were digested, washed with cold PBS, and resuspended in binding buffer, according to the instructions of the apoptosis kit. PE Annexin V and 7-amino-actinomycin (7-AAD; BD Pharmingen, San Diego, CA, USA) were added to the fixed cells for 15 min in the dark at room temperature. Then, Annexin V binding buffer was added to the mixture before the fluorescence was measured with an LSR II flow cytometer (BD, USA). Cell apoptosis was analyzed using FlowJo software (FlowJo, LLC, Bethesda, USA). Three separate experiments were performed.
Copyright and License information: ©2020 Cui, Song, Han, Hu, Chen, Chen, Xu, Xing, Lu and Cai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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