The human PC cell line LNCaP was obtained from American Type Culture Collection (Manassas, VA). LNCaP was cultured in RPMI-1640 supplemented with 10% FBS. LNCaP95 is previously established CRPC cell line expressing AR-V7 derived from LNCaP [16]. LNCaP95 was cultured in phenol red-free RPMI-1640 supplemented with 10% charcoal-stripped serum.
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