The cell proliferation assay using The IncuCyte® S3 Live-Cell Analysis system (Essen BioSciences Inc, USA), as described by Kalimutho et al.18. Doubling time was analyzed at every 12 h interval by counting the overall cell population compared to the originally seeded population using the Countess® automated cell counter (Life TechnologiesTM). The NIH-3T3 proliferation assay was performed by using the standard protocol43.
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