Cell proliferation assay

DS Debottam Sinha
PN Purba Nag
DN Devathri Nanayakkara
PD Pascal H. G. Duijf
AB Andrew Burgess
PR Prahlad Raninga
VS Veronique A. J. Smits
AB Amanda L. Bain
GS Goutham Subramanian
MW Meaghan Wall
JF John. W. Finnie
MK Murugan Kalimutho
KK Kum Kum Khanna
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The cell proliferation assay using The IncuCyte® S3 Live-Cell Analysis system (Essen BioSciences Inc, USA), as described by Kalimutho et al.18. Doubling time was analyzed at every 12 h interval by counting the overall cell population compared to the originally seeded population using the Countess® automated cell counter (Life TechnologiesTM). The NIH-3T3 proliferation assay was performed by using the standard protocol43.

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