2.4. Standard Histological Analysis

Histological analyses were performed in 5 µm cross sections from frozen mouse livers. Liver architecture was assessed by hematoxylin and eosin staining. Lipid content was measured using Oil Red O stain following the protocol described by Mehlem et al. [34]. Fibrosis was measured by Sirius red staining. Photographs were obtained using an optical microscope (Olympus IX71, Barcelona, Spain).