Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Hela cells were transfected with His-tagged ubiquitin, HA-tagged c-Myc without or with Gfi1 using Mirus Transfection Reagent. After incubation for 30 h, cells were treated with MG132 (40 µM) for 6 h and lysed in SDS lysis buffer. Samples were boiled for 10 min, sonicated briefly and diluted 1:10 with dilution buffer. After centrifugation, samples were subjected to immunoprecipitation using the anti-HA antibody under denaturing conditions prior to Western blot analysis.
Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
